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Human Pim-1 Oncogene (PIM1) ELISA Kit

SKU:
HUDL02294
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11309
Sensitivity:
0.25ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Pim-1 Oncogene (PIM1) ELISA Kit

The Human PIM-1 Oncogene (PIM1) ELISA Kit is specifically designed for the precise measurement of PIM1 levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research endeavors.The PIM-1 oncogene is a key player in cell proliferation and survival, playing a pivotal role in tumorigenesis and cancer progression. By detecting PIM1 levels, researchers can gain valuable insights into cancer development and identify potential therapeutic targets for intervention.

This ELISA kit from Assay Genie provides researchers with a reliable and efficient method for analyzing PIM1 levels, allowing for the exploration of its role in cancer biology and the development of novel treatment strategies. Upgrade your research capabilities with the Human PIM-1 Oncogene ELISA Kit today.

Product Name:Human Pim-1 Oncogene (PIM1) ELISA Kit
Product Code:HUDL02294
Size:96 Assays
Target:PIM1
Synonyms:Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1
Detection Method:Sandwich
Range:0.78-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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