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Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit (SBRS1989)

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SKU:
SBRS1989
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit SBRS1989
  • Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit SBRS1989
€1,089

Description

Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit (SBRS1989)

Product SKU:SBRS1989
Size:96T
Application:This ELISA kit recognizes human VEGFR2 phosphotyrosine.
Uniprot:Q14765
Gene ID:6775
Gene Names:STAT4
Pathway:JAK/STAT Signaling
Synonyms:Signal transducer and activator of transcription 4
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Biotinylated Anti-Phosphotyrosine Antibody
  4. Stop Solution
  5. Assay Diluent(s)
  6. Positive Control Sample
  7. Lysis Buffer
  8. Streptavidin-Conjugated HRP
  9. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit (SBRS1989) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageA431 cells were treated or untreated with EGF. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageHeLa cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 107 cells/ml in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA

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