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Human Phosphorylation Pathway Profiling Array (55 targets) (SARB0063)

SKU:
SARB0063
Product Type:
Phosphorylation Array
Analytes:
4E-BP1 (P-Thr36)
AKT (P-Ser473)
AMPKa (P-Thr172)
ATF2 (P-Thr69/71)
ATM (P-Ser1981)
BAD (P-Ser112)
C-Fos (P-Thr232)
c-Jun (P-Ser73)
CREB (P-Ser133)
EGFR (P-Ser1070)
eIF-2a (P-Ser51)
ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
GSK-3 alpha (P-Ser21)
GSK-3 beta (P-Ser9)
HDAC2 (P-Ser394)
HDAC4 (P-Ser632)
HSP27 (P-Ser82)
IKBa (P-Ser32)
JAK1 (P-Tyr1022)
JAK2 (P-Tyr1007/1008)
JNK (P-Thr183)
MEK (P-Ser217/221)
MKK3 (P-Ser189)
MKK6 (P-Ser207) (MEK6)
MSK1 (P-Ser376)
MSK2 (P-Ser360)
mTOR (P-Ser2448)
NF-kB (P-Ser536)
p27 (P-Thr198)
p38 (P-Thr180/Tyr182)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
PDK1 (P-Ser241)
PRAS40 (P-Thr246)
PTEN (P-Ser380)
Raf-1 (P-Ser301)
RPS6 (P-Ser235/236)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
SHP-1 (P-Ser591)
SHP2 (P-Tyr542)
SMAD1 (P-Ser463/465)
SMAD2 (P-Ser245/250/255)
SMAD4 (P-Thr277)
Reactivity:
Human
Applications:
Multiplex Array
€1,479 - €3,089
Frequently bought together:

Description

system_update_altDatasheet

Human Phosphorylation Pathway Profiling Array (55 targets) (SARB0063)

The Human Phosphorylation Pathway Profiling Array (SARB0063) is a powerful tool for researchers studying cellular signaling pathways involved in phosphorylation events. This array contains 55 key targets related to phosphorylation, allowing for comprehensive analysis of signaling cascades in a high-throughput manner.Each target on the array is carefully selected to provide insight into the phosphorylation status of crucial proteins involved in various signaling pathways. By using this array, researchers can profile the phosphorylation levels of multiple targets simultaneously, enabling a deeper understanding of how these pathways are regulated in different experimental conditions.

This array is compatible with human samples, making it a valuable resource for studies in cell signaling, cancer biology, and drug discovery. By utilizing the Human Phosphorylation Pathway Profiling Array, researchers can uncover new signaling nodes, identify potential therapeutic targets, and elucidate the mechanisms underlying various diseases and biological processes.

Product Sku:SARB0063
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:55
Gene Symbols:AKT1, AKT1S1, AMPK1, AND, ATF2, ATM, BAD, CDKN1B, CREB1, EGFR|EIF2S1, EIF4EBP1, FOS, GSK3A, GSK3B, HDAC2, HDAC4, HSPB1, JAK1, JAK2, JUN, MAP2K1, MAP2K3, MAP2K6, MAP3K7, MAPK14, MAPK8, MTOR, NFKBIA, PDPK1, PTEN, PTPN11, PTPN6, RAF1, RELA, RPS6, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KA5, RPS6KB1, SMAD1, SMAD2, SMAD4, SMAD5, SRC, STAT1, STAT2, STAT3, STAT5A, STAT6, TBK1, TP53, TYK2, ZAP70
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / TGF-beta Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
4E-BP1 (P-Thr36)
  • Gene Symbol: EIF4EBP1
  • Gene ID: 1978
  • Accession #: Q13541
AKT (P-Ser473)
  • Gene Symbol: Akt1 Akt Rac
  • Gene ID: 11651
  • Accession #: P31750
AMPKa (P-Thr172)
  • Gene Symbol: AMPK1
  • Gene ID: 5562
  • Accession #: P54645
ATF2 (P-Thr69/71)
  • Gene Symbol: ATF2 CREB2 CREBP1
  • Gene ID: 1386;
  • Accession #: P15336
ATM (P-Ser1981)
  • Gene Symbol: ATM
  • Gene ID: 472;
  • Accession #: Q13315
BAD (P-Ser112)
  • Gene Symbol: Bad Bbc6
  • Gene ID: 12015
  • Accession #: Q61337
C-Fos (P-Thr232)
  • Gene Symbol: FOS G0S7
  • Gene ID: 2353;
  • Accession #: P01100
c-Jun (P-Ser73)
  • Gene Symbol: JUN
  • Gene ID: 3725;
  • Accession #: P05412
CREB (P-Ser133)
  • Gene Symbol: CREB1
  • Gene ID: 1385
  • Accession #: P16220
EGFR (P-Ser1070)
  • Gene Symbol: EGFR ERBB ERBB1 HER1
  • Gene ID: 1956
  • Accession #: P00533
eIF-2a (P-Ser51)
  • Gene Symbol: EIF2S1 EIF2A
  • Gene ID: 1965;
  • Accession #: P05198
ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
  • Gene Symbol: AND ERK1 ERK2 MAPK1 MAPK3 PRKM1 PRKM2 PRKM3
  • Gene ID: 5595 5594
  • Accession #: P27361 P28482
GSK-3 alpha (P-Ser21)
  • Gene Symbol: GSK3A
  • Gene ID: 2931
  • Accession #: P49840
GSK-3 beta (P-Ser9)
  • Gene Symbol: GSK3B
  • Gene ID: 2932;
  • Accession #: P49841
HDAC2 (P-Ser394)
  • Gene Symbol: HDAC2
  • Gene ID: 3066
  • Accession #: Q92769
HDAC4 (P-Ser632)
  • Gene Symbol: HDAC4 KIAA0288
  • Gene ID: 9759;
  • Accession #: P56524
HSP27 (P-Ser82)
  • Gene Symbol: Hspb1 Hsp25 Hsp27
  • Gene ID: 15507
  • Accession #: P14602
IKBa (P-Ser32)
  • Gene Symbol: Nfkbia Ikba
  • Gene ID: 18035
  • Accession #: Q9Z1E3
JAK1 (P-Tyr1022)
  • Gene Symbol: JAK1 JAK1A JAK1B
  • Gene ID: 3716
  • Accession #: P23458
JAK2 (P-Tyr1007/1008)
  • Gene Symbol: JAK2
  • Gene ID: 3717
  • Accession #: O60674
JNK (P-Thr183)
  • Gene Symbol: Mapk8 Jnk1 Prkm8
  • Gene ID: 26419
  • Accession #: Q91Y86
MEK (P-Ser217/221)
  • Gene Symbol: MAP2K1 MEK1 PRKMK1
  • Gene ID: 5604
  • Accession #: Q02750
MKK3 (P-Ser189)
  • Gene Symbol: MAP2K3 MEK3 MKK3 PRKMK3 SKK2
  • Gene ID: 5606
  • Accession #: P46734
MKK6 (P-Ser207) (MEK6)
  • Gene Symbol: MAP2K6 MEK6 MKK6 PRKMK6 SKK3
  • Gene ID: 5608
  • Accession #: P52564
MSK1 (P-Ser376)
  • Gene Symbol: RPS6KA5 MSK1
  • Gene ID: 9252;
  • Accession #: O75582
MSK2 (P-Ser360)
  • Gene Symbol: RPS6KA4 MSK2
  • Gene ID: 8986
  • Accession #: O75676
mTOR (P-Ser2448)
  • Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1
  • Gene ID: 2475
  • Accession #: P42345
NF-kB (P-Ser536)
  • Gene Symbol: RELA NFKB3
  • Gene ID: 5970
  • Accession #: Q04206
p27 (P-Thr198)
  • Gene Symbol: Cdkn1b
  • Gene ID: 12576
  • Accession #: P46414
p38 (P-Thr180/Tyr182)
  • Gene Symbol: Mapk14 Crk1 Csbp1 Csbp2
  • Gene ID: 26416
  • Accession #: P47811
P53 (P-Ser15)
  • Gene Symbol: Tp53 P53 Trp53
  • Gene ID: 22059
  • Accession #: P02340
P70S6K (P-Thr421/Ser424)
  • Gene Symbol: RPS6KB1 STK14A
  • Gene ID: 6198
  • Accession #: P23443
PDK1 (P-Ser241)
  • Gene Symbol: PDPK1 PDK1
  • Gene ID: 5170
  • Accession #: O15530
PRAS40 (P-Thr246)
  • Gene Symbol: AKT1S1 PRAS40
  • Gene ID: 84335
  • Accession #: Q96B36
PTEN (P-Ser380)
  • Gene Symbol: PTEN MMAC1 TEP1
  • Gene ID: 5728
  • Accession #: P60484
Raf-1 (P-Ser301)
  • Gene Symbol: RAF1 RAF
  • Gene ID: 5894
  • Accession #: P04049
RPS6 (P-Ser235/236)
  • Gene Symbol: RPS6 OK/SW-cl.2
  • Gene ID: 6194
  • Accession #: P62753
RSK1 (P-Ser380)
  • Gene Symbol: RPS6KA1 MAPKAPK1A RSK1
  • Gene ID: 6195
  • Accession #: Q15418
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 6197
  • Accession #: P51812
SHP-1 (P-Ser591)
  • Gene Symbol: PTPN6 HCP PTP1C
  • Gene ID: 5777
  • Accession #: P29350
SHP2 (P-Tyr542)
  • Gene Symbol: PTPN11 PTP2C SHPTP2
  • Gene ID: 5781
  • Accession #: Q06124
SMAD1 (P-Ser463/465)
  • Gene Symbol: SMAD1 BSP1 MADH1 MADR1
  • Gene ID: 4086;
  • Accession #: Q15797
SMAD2 (P-Ser245/250/255)
  • Gene Symbol: SMAD2 MADH2 MADR2
  • Gene ID: 4087;
  • Accession #: Q15796
SMAD4 (P-Thr277)
  • Gene Symbol: SMAD4 DPC4 MADH4
  • Gene ID: 4089;
  • Accession #: Q13485
SMAD5 (P-Ser463/465)
  • Gene Symbol: SMAD5 MADH5
  • Gene ID: 4090;
  • Accession #: Q99717
Src (P-Tyr419)
  • Gene Symbol: SRC SRC1
  • Gene ID: 6714
  • Accession #: P12931
STAT1 (P-Ser727)
  • Gene Symbol: STAT1
  • Gene ID: 6772
  • Accession #: P42224
STAT2 (P-Tyr689)
  • Gene Symbol: STAT2
  • Gene ID: 6773
  • Accession #: P52630
STAT3 (P-Tyr705)
  • Gene Symbol: STAT3 APRF
  • Gene ID: 6774
  • Accession #: P40763
STAT5 (P-Tyr694)
  • Gene Symbol: STAT5A STAT5
  • Gene ID: 6776
  • Accession #: P42229
STAT6 (P-Tyr641)
  • Gene Symbol: STAT6
  • Gene ID: 6778
  • Accession #: P42226
TAK1 (P-Ser412)
  • Gene Symbol: MAP3K7 TAK1
  • Gene ID: 6885;
  • Accession #: O43318
TBK1 (P-Ser172)
  • Gene Symbol: TBK1 NAK
  • Gene ID: 29110;
  • Accession #: Q9UHD2
TYK2 (P-Tyr1054)
  • Gene Symbol: TYK2
  • Gene ID: 7297
  • Accession #: P29597
ZAP70 (P-Tyr292)
  • Gene Symbol: ZAP70 SRK
  • Gene ID: 7535;
  • Accession #: P43403
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Phosphorylation Pathway Profiling Array C55 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 500X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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