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Human Phospholipase C Gamma 1 (PLCg1) ELISA Kit

SKU:
HUDL02328
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19174
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NCKAP3,PLC-G1,PLC-II,PLC1,PLC148,PLCgamma1,1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1,Phosphoinositide phospholipase C-gamma-1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Phospholipase C Gamma 1 (PLCg1) ELISA Kit

The Human Phospholipase C gamma 1 (PLCG1) ELISA Kit is specially designed for the precise measurement of PLCG1 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Phospholipase C gamma 1 is a key enzyme involved in cell signaling pathways, particularly in response to growth factors and immune signals. Dysregulation of PLCG1 has been implicated in various diseases, including cancer, autoimmune disorders, and neurological conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.

With the Human PLCG1 ELISA Kit, researchers can confidently analyze PLCG1 levels in biological samples, gaining insights into its role in health and disease. Trust in this kit for reliable and reproducible data, advancing your research in the field of cell signaling and disease mechanisms.

Product Name:Human Phospholipase C Gamma 1 (PLCg1) ELISA Kit
Product Code:HUDL02328
Size:96 Assays
Target:PLCg1
Synonyms:NCKAP3, PLC-G1, PLC-II, PLC1, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, Phosphoinositide phospholipase C-gamma-1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)85-10092
EDTA plasma (n=5)85-10394
Heparin plasma (n=5)87-9993
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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