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Human Phospholipase A2 Activating Protein (PLAA) ELISA Kit

SKU:
HUDL02324
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y263
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
PLAP,PLA2P,PLA2-P,DOA1
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Human Phospholipase A2 Activating Protein (PLAA) ELISA Kit

The Human Phospholipase A2 Activating Protein (PLAA) ELISA Kit is specifically designed for the precise measurement of PLAA levels in human serum, plasma, and cell culture supernatants. This kit offers unparalleled accuracy and reliability, ensuring consistent and reproducible results for a variety of research applications. PLAA is a vital protein that plays a key role in activating phospholipase A2, which is involved in various biological processes such as lipid metabolism and inflammation.

Dysregulation of PLAA has been associated with inflammatory diseases, neurological disorders, and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. With its high sensitivity and specificity, the Human PLAA ELISA Kit is a valuable tool for researchers aiming to investigate the role of PLAA in health and disease. Stay ahead of the curve in your research with this innovative and reliable ELISA kit from Assaygenie.

Product Name:Human Phospholipase A2 Activating Protein (PLAA) ELISA Kit
Product Code:HUDL02324
Size:96 Assays
Target:PLAA
Synonyms:PLAP, PLA2P, PLA2-P, DOA1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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