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Human Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) ELISA Kit

SKU:
HUDL02292
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P42338
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PI3K,PI3Kbeta,PIK3C1,p110-BETA,Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) ELISA Kit

The Human Phosphoinositide 3-Kinase Catalytic Beta Polypeptide (PIK3CB) ELISA Kit is designed for the precise measurement of PIK3CB levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.PIK3CB is a key enzyme in the PI3K signaling pathway, playing a critical role in cell growth, proliferation, and survival.

Dysregulation of PIK3CB has been linked to various diseases, including cancer, diabetes, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With its reliable performance and ease of use, the Human PIK3CB ELISA Kit is a valuable tool for researchers seeking to investigate the role of PIK3CB in disease pathogenesis and identify potential therapeutic targets.

Product Name:Human Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) ELISA Kit
Product Code:HUDL02292
Size:96 Assays
Target:PIK3Cb
Synonyms:PI3K, PI3Kbeta, PIK3C1, p110-BETA, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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