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Human Phosphofructokinase, Platelet (PFKP) ELISA Kit

SKU:
HUDL02267
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q01813
Sensitivity:
0.136ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
PFK-C,PFKF,ATP-dependent 6-phosphofructokinase, platelet type,6-phosphofructokinase type C,Phosphofructo-1-kinase isozyme C,Phosphohexokinase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Phosphofructokinase, Platelet (PFKP) ELISA Kit

The Human Phosphofructokinase Platelet (PFKP) ELISA Kit is specifically designed for the precise detection of PFKP levels in human serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.PFKP is a key enzyme in glycolysis, playing a critical role in cellular energy production. Dysregulation of PFKP has been linked to various diseases such as cancer, metabolic disorders, and neurodegenerative conditions.

Therefore, the measurement of PFKP levels can provide valuable insights into disease pathogenesis and potential therapeutic interventions.Overall, the Human PFKP ELISA Kit is a valuable tool for researchers studying metabolic pathways, disease mechanisms, and developing targeted treatments. With its reliable performance and ease of use, this kit is an essential addition to any laboratory conducting groundbreaking research in the field of molecular biology.

Product Name:Human Phosphofructokinase, Platelet (PFKP) ELISA Kit
Product Code:HUDL02267
Size:96 Assays
Target:PFKP
Synonyms:PFK-C, PFKF, ATP-dependent 6-phosphofructokinase, platelet type, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, Phosphohexokinase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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