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Human Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit

SKU:
HUDL02207
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35558
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PEPCK1,PEPCKC,Cytosolic Phosphoenolpyruvate Carboxykinase,Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit

The Human Phosphoenolpyruvate Carboxykinase 1 (soluble PCK1) ELISA Kit is specifically designed for the accurate measurement of soluble PCK1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Soluble PCK1 is a key enzyme involved in gluconeogenesis, playing a crucial role in regulating glucose metabolism. Dysregulation of PCK1 has been linked to metabolic disorders such as diabetes and obesity, making it a valuable biomarker for studying these conditions and potential therapeutic targets.

With its advanced technology and user-friendly design, the Human PCK1 ELISA Kit is a valuable tool for researchers investigating glucose metabolism, metabolic disorders, and potential therapeutic interventions. Trust in the accuracy and reliability of this kit for your research needs.

Product Name:Human Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit
Product Code:HUDL02207
Size:96 Assays
Target:PCK1
Synonyms:PEPCK1, PEPCKC, Cytosolic Phosphoenolpyruvate Carboxykinase, Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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