Human Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit
The Human Phosphodiesterase 3B (cGMP-inhibited PDE3B) ELISA Kit is a powerful tool for accurately measuring levels of phosphodiesterase 3B in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Phosphodiesterase 3B is a key enzyme involved in cellular signaling pathways, particularly in the regulation of cGMP levels. Dysregulation of PDE3B has been implicated in various diseases including diabetes, cardiovascular disorders, and certain types of cancer.
By accurately measuring PDE3B levels, researchers can gain valuable insights into these conditions and potentially identify novel therapeutic targets.Overall, the Human Phosphodiesterase 3B (cGMP-inhibited PDE3B) ELISA Kit is a valuable tool for researchers looking to study the role of PDE3B in health and disease, providing accurate and reliable data to advance scientific understanding and potentially improve patient outcomes.
Product Name:
Human Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit
Product Code:
HUDL02231
Size:
96 Assays
Target:
PDE3B
Synonyms:
HcGIP1, cGIPDE1, CGIPDE1, CGIP1, CGI-PDE B, Cyclic GMP-inhibited phosphodiesterase B
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
