Human Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) ELISA Kit
The Human Phosphodiesterase 1C Calmodulin-Dependent (PDE1C) ELISA Kit is designed for the accurate and sensitive detection of PDE1C levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with a reliable tool for studying the enzymatic activity of PDE1C, a key regulator of intracellular cyclic nucleotide signaling pathways.PDE1C is a crucial enzyme involved in various physiological processes, including cell growth, differentiation, and neuronal function. Dysregulation of PDE1C has been associated with diseases such as cardiovascular disorders, neurological disorders, and cancer, highlighting the importance of studying its activity and expression levels.
With high sensitivity and specificity, the Human PDE1C ELISA Kit offers accurate and reproducible results, enabling researchers to explore the potential role of PDE1C in disease pathology and identify potential therapeutic targets. This kit is suitable for a wide range of research applications and can aid in advancing our understanding of PDE1C biology and its implications in human health.
Product Name:
Human Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
