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Human Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) ELISA Kit

SKU:
HUDL02228
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P54750
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
hCam-1,61 kDa Cam-PDE,Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) ELISA Kit

The Human Phosphodiesterase 1A (Calmodulin-dependent PDE1A) ELISA Kit is a powerful tool for the accurate measurement of PDE1A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PDE1A is a critical enzyme involved in the regulation of intracellular levels of cyclic nucleotides, playing a vital role in various physiological processes such as cell signaling, smooth muscle contraction, and neuronal function.

Dysregulation of PDE1A has been implicated in a range of diseases including cardiovascular disorders, neurological conditions, and cancer, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.With its high-performance capabilities and ease of use, the Human Phosphodiesterase 1A (Calmodulin-dependent PDE1A) ELISA Kit is an essential tool for researchers seeking to explore the role of PDE1A in health and disease.

Product Name:Human Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) ELISA Kit
Product Code:HUDL02228
Size:96 Assays
Target:PDE1A
Synonyms:hCam-1, 61 kDa Cam-PDE, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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