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Human Phosphodiesterase 12 (PDE12) ELISA Kit

SKU:
HUDL02227
Product Type:
ELISA Kit
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
2'-PDE,2'-Phosphodiesterase,Mitochondrial deadenylase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Phosphodiesterase 12 (PDE12) ELISA Kit

The Human Phosphodiesterase 12 (PDE12) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PDE12 levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a wide range of research applications.PDE12 is an important enzyme involved in the regulation of cyclic nucleotide levels, playing a critical role in various cellular processes. Dysregulation of PDE12 has been associated with neurological disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

With its high sensitivity and accuracy, the Human Phosphodiesterase 12 (PDE12) ELISA Kit is an essential tool for researchers seeking to investigate the role of PDE12 in health and disease. Get your kit today and unlock new insights into the function of this important enzyme.

Product Name:Human Phosphodiesterase 12 (PDE12) ELISA Kit
Product Code:HUDL02227
Size:96 Assays
Target:PDE12
Synonyms:2'-PDE, 2'-Phosphodiesterase, Mitochondrial deadenylase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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