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Human Phospho-Estrogen receptor (S118) and Total Estrogen receptor PharmaGenie ELISA Kit (SBRS1814)

SKU:
SBRS1814
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human Phospho-Estrogen receptor S118 and Total Estrogen receptor PharmaGenie ELISA Kit SBRS1814
  • Human Phospho-Estrogen receptor S118 and Total Estrogen receptor PharmaGenie ELISA Kit SBRS1814
$984
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Description

Human Phospho-Estrogen receptor (S118) and Total Estrogen receptor PharmaGenie ELISA Kit (SBRS1814)

Product SKU:SBRS1814
Size:96T
Application:The antibody pair provided in this kit recognizes Human Estrogen receptor phosphorylated at site Serine-118 as well as total Estrogen receptor.
Uniprot:P03372
Gene ID:2099
Gene Names:ESR1
Pathway:MAPK Signaling
Synonyms:Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-Estrogen receptor (S118) and Total Estrogen receptor PharmaGenie ELISA Kit (SBRS1814) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageZR751 cells were untreated or treated with b-estradiol for 1 hour. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageZR751 cells were treated with b-estradiol at 37°C for 1 hours. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA

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