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Human Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit

SKU:
HUDL02255
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P30086
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PBP,HCNP,PEBP,RKIP,Prostatic Binding Protein,Raf Kinase Inhibitory Protein,Hippocampal Cholinergic Neurostimulating Peptide,Neuropolypeptide h3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit

The Human Phosphatidylethanolamine-binding protein 1 (PEBP1) ELISA Kit is a reliable tool for the accurate measurement of PEBP1 levels in human biological samples. This kit offers high sensitivity and specificity, ensuring robust and consistent results for various research applications.PEBP1 is a vital protein that plays a role in cellular processes such as cell growth, survival, and signaling. It is implicated in various diseases including cancer, neurological disorders, and inflammatory conditions, making it a valuable biomarker for research and therapeutic development.

With its user-friendly protocol and efficient performance, the Human PEBP1 ELISA Kit is an essential tool for scientists and researchers studying the role of PEBP1 in health and disease. Obtain precise and reliable data with this advanced ELISA kit for high-quality research outcomes.

Product Name:Human Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit
Product Code:HUDL02255
Size:96 Assays
Target:PEBP1
Synonyms:PBP, HCNP, PEBP, RKIP, Prostatic Binding Protein, Raf Kinase Inhibitory Protein, Hippocampal Cholinergic Neurostimulating Peptide, Neuropolypeptide h3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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