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Human Phosphatase And Tensin Homolog (PTEN) ELISA Kit

SKU:
HUDL02441
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P60484
Sensitivity:
0.136ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
BZS,MHAM,MMAC1,PTEN1,TEP1,Mutated In Multiple Advanced Cancers 1,Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Human Phosphatase And Tensin Homolog (PTEN) ELISA Kit

The Human Phosphatase and Tensin Homolog (PTEN) ELISA Kit is specifically designed for the precise quantification of PTEN levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.PTEN is a critical tumor suppressor gene that plays a key role in regulating cell growth, proliferation, and survival.

Dysregulation of PTEN expression has been linked to numerous diseases, including cancer, neurological disorders, and metabolic syndromes, highlighting its importance as a biomarker for disease diagnosis and therapeutic development.With this comprehensive ELISA kit, researchers can confidently measure PTEN levels in a simple and efficient manner, facilitating groundbreaking discoveries in the field of molecular biology and personalized medicine.

Product Name:Human Phosphatase And Tensin Homolog (PTEN) ELISA Kit
Product Code:HUDL02441
Size:96 Assays
Target:PTEN
Synonyms:BZS, MHAM, MMAC1, PTEN1, TEP1, Mutated In Multiple Advanced Cancers 1, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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