The Human PGR (Progesterone Receptor) ELISA Kit is specifically designed for the precise measurement of progesterone receptor levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it well-suited for various research applications.The progesterone receptor is a critical protein involved in mediating the effects of progesterone, a hormone essential for reproductive function and pregnancy maintenance.
Dysregulation of progesterone receptor signaling has been implicated in various diseases such as breast cancer, endometrial cancer, and other reproductive disorders, highlighting its importance as a biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human PGR ELISA Kit from Assay Genie provides researchers with a valuable tool for studying progesterone receptor biology and its role in health and disease.
Product Name:
Human PGR (Progesterone Receptor) ELISA Kit
SKU:
HUES03154
Target:
Human PGR (Progesterone Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PGR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PGR. You can calculate the concentration of Human PGR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
86-99
86-99
Average (%)
91
93
93
1:4
Range (%)
90-104
88-98
87-97
Average (%)
96
93
92
1:8
Range (%)
90-101
83-97
85-99
Average (%)
95
89
91
1:16
Range (%)
91-107
82-94
84-97
Average (%)
98
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.64
7.33
0.94
2.4
7.41
Standard deviation
0.05
0.15
0.29
0.06
0.14
0.28
C V (%)
5.21
5.68
3.96
6.38
5.83
3.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PGR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGR in samples. No significant cross-reactivity or interference between Human PGR and analogues was observed.
