Human PGD Recombinant Protein (RPPB2100)
- SKU:
- RPPB2100
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P52209
- Research Area:
- Enzymes
Description
| Product Name: | Human PGD Recombinant Protein |
| Product Code: | RPPB2100 |
| Size: | 10µg |
| Species: | Human |
| Target: | PGD |
| Synonyms: | EC 1.1.1.44, 6PGD, PGDH, 6-phosphogluconate dehydrogenase decarboxylating, PGD. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PGD solution (1mg/ml) contains 10% Glycerol, 1mM DTT, 0.1M NaCl, and 20mM Tris-HCl buffer (pH 8.0). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL�LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF�GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA�� |
| Biological Activity: | Specific activity is > 10unit/mg. One unit will oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5- phosphate per minute at pH 8.0 at 25?C, in the presence of beta-NADP. |
6PGD is the 2nd dehydrogenase in the pentose phosphate shift. Pentose is crucial for the biosynthesis of nucleic acid. The pentose phosphate cycle is a prominent source of NADPH. 6PGD deficiency is mostly asymptomatic, and the inheritance of this deasis is autosomal dominant. PGD deficiency elevate the erythrocyte pyruvate kinase levels of activity & decreases glutathione synthetase, which causes hemolysis.
PGD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 503 amino acids (1-483) and having a molecular mass of 55.3 kDa.PGD Human is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PGD: an oxidative carboxylase that catalyses the decarboxylating reduction of 6-phosphogluconate into ribulose 5-phosphate in the presence of NADP. This reaction is a component of the hexose mono-phosphate shunt and pentose phosphate pathways. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pentose phosphate pathway; EC 1.1.1.44; Oxidoreductase; Other Amino Acids Metabolism - glutathione Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cytosol; nucleus Molecular Function:phosphogluconate dehydrogenase (decarboxylating) activity; NADP binding Biological Process: pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch; carbohydrate metabolic process; pathogenesis; D-gluconate metabolic process; pentose biosynthetic process |
| NCBI Summary: | 6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015] |
| UniProt Code: | P52209 |
| NCBI GenInfo Identifier: | 40068518 |
| NCBI Gene ID: | 5226 |
| NCBI Accession: | NP_002622.2 |
| UniProt Secondary Accession: | P52209,Q9BWD8, A8K2Y9, B4DQJ8, |
| UniProt Related Accession: | P52209 |
| Molecular Weight: | 51,872 Da |
| NCBI Full Name: | 6-phosphogluconate dehydrogenase, decarboxylating isoform 1 |
| NCBI Synonym Full Names: | phosphogluconate dehydrogenase |
| NCBI Official Symbol: | PGD�� |
| NCBI Official Synonym Symbols: | 6PGD�� |
| NCBI Protein Information: | 6-phosphogluconate dehydrogenase, decarboxylating |
| UniProt Protein Name: | 6-phosphogluconate dehydrogenase, decarboxylating |
| UniProt Gene Name: | PGD�� |
| UniProt Entry Name: | 6PGD_HUMAN |
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