Human PGC Recombinant Protein (RPPB2116)
- SKU:
- RPPB2116
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P20142
- Research Area:
- Enzymes
Description
| Product Name: | Human PGC Recombinant Protein |
| Product Code: | RPPB2116 |
| Size: | 5µg |
| Species: | Human |
| Target: | PGC |
| Synonyms: | Progastricsin (Pepsinogen C), Pepsinogen C, EC 3.4.23.3,Pepsinogen Group II, Preprogastricsin, EC 3.4.23, Pepsin C, PGII, PEPC. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PGC protein solution (0.25mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | AVVKVPLKKF KSIRETMKEK GLLGEFLRTH KYDPAWKYRF GDLSVTYEPMAYMDAAYFGE ISIGTPPQNF LVLFDTGSSN LWVPSVYCQS QACTSHSRFN PSESSTYSTN GQTFSLQYGSGSLTGFFGYD TLTVQSIQVP NQEFGLSENE PGTNFVYAQF DGIMGLAYPA LSVDEATTAM QGMVQEGALTSPVFSVYLSN QQGSSGGAVV FGGVDSSLYT GQIYWAPVTQ ELYWQIGIEE FLIGGQASGW CSEGCQAIVDTGTSLLTVPQ QYMSALLQAT GAQEDEYGQF LVNCNSIQNL PSLTFIINGV EFPLPPSSYI LSNNGYCTVGVEPTYLSSQN GQPLWILGDV FLRSYYSVYD LGNNRVGFAT AALEHHHHHH |
Progastricsin-C(PGC) is an aspartic proteinase which is synthesized in the gastric mucosa asinactive precursors. PGC is a part of the peptidase family A1 and contains aprosegment which is responsible for stabilizing the inactive form and preventingthe entrance of the substrate to the active site. PGC is used as a biomarkerfor various gastric diseases including Helicobacter pylori related gastritis. PGCis also hydrolyzes various proteins.
PGC produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 380 amino acids (17-388 a.a.) andhaving a molecular mass of 41.6kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).�PGC is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PGC: Hydrolyzes a variety of proteins. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Protease; Secreted; EC 3.4.23.3 Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: extracellular space Molecular Function:aspartic-type endopeptidase activity Biological Process: digestion; proteolysis |
| NCBI Summary: | This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009] |
| UniProt Code: | P20142 |
| NCBI GenInfo Identifier: | 129796 |
| NCBI Gene ID: | 5225 |
| NCBI Accession: | P20142.1 |
| UniProt Secondary Accession: | P20142,Q5T3D7, Q5T3D8, B4DVZ3, |
| UniProt Related Accession: | P20142 |
| Molecular Weight: | 40,600 |
| NCBI Full Name: | Gastricsin |
| NCBI Synonym Full Names: | progastricsin (pepsinogen C) |
| NCBI Official Symbol: | PGC�� |
| NCBI Official Synonym Symbols: | PEPC; PGII�� |
| NCBI Protein Information: | gastricsin; pepsin C; pepsinogen C; preprogastricsin; pepsinogen group II |
| UniProt Protein Name: | Gastricsin |
| UniProt Synonym Protein Names: | Pepsinogen C |
| Protein Family: | Gastricsin |
| UniProt Gene Name: | PGC�� |
| UniProt Entry Name: | PEPC_HUMAN |
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