Human PGAM1 Recombinant Protein (RPPB2094)
- SKU:
- RPPB2094
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P18669
- Research Area:
- Enzymes
Description
| Product Name: | Human PGAM1 Recombinant Protein |
| Product Code: | RPPB2094 |
| Size: | 10µg |
| Species: | Human |
| Target: | PGAM1 |
| Synonyms: | Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | The PGAM1 1mg/ml protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK |
| Biological Activity: | Specific activity is >300 units/mg, in which One unitwill convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute atpH 7.6 at 37C. |
PGAM1 is part of the phosphoglycerate mutase family. PGAM1 is an essential component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. PGAM1 is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). PGAM1 mutations lead to muscle phosphoglycerate mutase deficiency, a.k.a. glycogen storage disease X.
PGAM1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 274 amino acids (1-254 a.a.) and having a molecular mass of 30.9 kDa. The PGAM1 is fused to a 20 amino acid His Tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. |
| UniProt Protein Details: | Protein type:EC 5.4.2.4; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; EC 3.1.3.13; Phosphatase (non-protein); EC 5.4.2.11 Chromosomal Location of Human Ortholog: 10q25.3 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; regulation of pentose-phosphate shunt; gluconeogenesis |
| NCBI Summary: | Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010] |
| UniProt Code: | P18669 |
| NCBI GenInfo Identifier: | 130348 |
| NCBI Gene ID: | 5223 |
| NCBI Accession: | P18669.2 |
| UniProt Secondary Accession: | P18669,Q9BWC0, |
| UniProt Related Accession: | P18669 |
| Molecular Weight: | 254 |
| NCBI Full Name: | Phosphoglycerate mutase 1 |
| NCBI Synonym Full Names: | phosphoglycerate mutase 1 (brain) |
| NCBI Official Symbol: | PGAM1�� |
| NCBI Official Synonym Symbols: | PGAMA; PGAM-B; HEL-S-35�� |
| NCBI Protein Information: | phosphoglycerate mutase 1; BPG-dependent PGAM 1; phosphoglycerate mutase isozyme B; epididymis secretory protein Li 35; phosphoglycerate mutase A, nonmuscle form |
| UniProt Protein Name: | Phosphoglycerate mutase 1 |
| UniProt Synonym Protein Names: | BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B |
| Protein Family: | Phosphoglycerate mutase |
| UniProt Gene Name: | PGAM1�� |
| UniProt Entry Name: | PGAM1_HUMAN |
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