The Human PFN1 (Profilin 1) ELISA Kit is specially designed for the accurate measurement of PFN1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results that are perfect for various research applications.PFN1, also known as Profilin 1, is a critical protein that plays a key role in actin dynamics and cell motility. It is involved in various cellular processes such as cell migration, division, and adhesion.
Dysregulation of PFN1 has been linked to diseases like cancer and neurodegenerative disorders, highlighting its importance as a biomarker in disease research and drug development.Overall, the Human PFN1 ELISA Kit is a valuable tool for researchers studying PFN1-related pathways and diseases, providing a reliable platform for measuring PFN1 levels with precision and accuracy.
Product Name:
Human PFN1 (Profilin 1) ELISA Kit
SKU:
HUES03179
Target:
Human PFN1 (Profilin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PFN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PFN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PFN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PFN1. You can calculate the concentration of Human PFN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
93-109
86-96
Average (%)
104
99
91
1:4
Range (%)
91-104
84-94
86-101
Average (%)
97
89
92
1:8
Range (%)
91-102
84-98
82-95
Average (%)
96
91
88
1:16
Range (%)
89-101
85-99
85-98
Average (%)
95
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
98
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
266.73
669.93
1854.65
241.85
717.33
1776.51
Standard deviation
13.55
33.5
83.09
12.5
35.44
93.44
C V (%)
5.08
5.0
4.48
5.17
4.94
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PFN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PFN1 in samples. No significant cross-reactivity or interference between Human PFN1 and analogues was observed.
