The Assay Genie Human PF4 (Platelet Factor 4) ELISA Kit is specifically developed to quantitatively measure the levels of Platelet Factor 4 (PF4) in a variety of biological samples. PF4, a chemokine protein released by platelets during the aggregation process, plays crucial roles in the regulation of blood coagulation, wound healing, and immune responses.
Accurate quantification of PF4 is essential for understanding its functions in platelet function, vascular biology, and immunomodulation. Our PF4 ELISA Kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs. Manufactured under strict quality control guidelines, this kit delivers robust performance and user-friendly protocols, making it an optimal choice for investigations focused on platelet biology, coagulation processes, and immune-related pathways. Trust in Assay Genie's PF4 ELISA Kit for dependable and accurate quantification of Platelet Factor 4 in your scientific endeavors.
Product Name:
Human PF4 (Platelet Factor 4) ELISA Kit
SKU:
AEES00184
Target:
Human PF4 (Platelet Factor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PF4. You can calculate the concentration of Human PF4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
96-108
88-100
Average (%)
91
101
94
1:4
Range (%)
86-97
93-105
85-98
Average (%)
91
98
90
1:8
Range (%)
86-100
91-104
88-102
Average (%)
93
98
95
1:16
Range (%)
86-100
97-110
87-102
Average (%)
92
102
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.08
3.72
15.4
1.88
3.9
16.3
Standard deviation
0.13
0.18
0.53
0.12
0.16
0.52
C V (%)
6.04
4.79
3.43
6.2
4.09
3.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PF4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PF4 in samples. No significant cross-reactivity or interference between Human PF4 and analogues was observed.
