null

Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ELISA Kit

SKU:
HUDL02374
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UBK2
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LEM6,PGC-1(alpha),PGC-1v,PGC1,PGC1A,PPARGC1,Ligand effect modulator 6
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ELISA Kit

The Human Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha (PPARGC1A) ELISA Kit is specifically designed for the accurate measurement of PPARGC1A levels in human serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PPARGC1A is a key regulator of energy metabolism and mitochondrial function, playing a crucial role in the regulation of various metabolic processes. Dysregulation of PPARGC1A has been linked to metabolic disorders, neurodegenerative diseases, and cardiovascular conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.

The Human PPARGC1A ELISA Kit provides researchers with a reliable tool for quantifying PPARGC1A levels, allowing for in-depth analysis of its role in disease pathology and the development of targeted treatments. Get accurate and meaningful results with this advanced ELISA kit from Assay Genie.

Product Name:Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ELISA Kit
Product Code:HUDL02374
Size:96 Assays
Target:PPARgC1a
Synonyms:LEM6, PGC-1(alpha), PGC-1v, PGC1, PGC1A, PPARGC1, Ligand effect modulator 6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products