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Human Peroxiredoxin 6 (PRDX6) ELISA Kit

SKU:
HUDL02395
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P30041
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
1-Cys,AOP2,NSGPx,PRX,aiPLA2,p29,Acidic calcium-independent phospholipase A2,Antioxidant protein 2,Non-selenium glutathione peroxidase,Red blood cells page spot 12
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Peroxiredoxin 6 (PRDX6) ELISA Kit

The Human Peroxiredoxin 6 (PRDX6) ELISA Kit is a powerful tool designed for the accurate and reliable detection of PRDX6 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, guaranteeing precise and reproducible results for a variety of research applications.PRDX6 is a vital enzyme involved in antioxidant defense and cell survival, playing a critical role in protecting cells from oxidative damage and regulating various cellular processes.

Dysregulation of PRDX6 has been linked to various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.In summary, the Human PRDX6 ELISA Kit offers researchers a reliable and accurate tool for studying the role of PRDX6 in health and disease, providing valuable insights into its potential as a therapeutic target.

Product Name:Human Peroxiredoxin 6 (PRDX6) ELISA Kit
Product Code:HUDL02395
Size:96 Assays
Target:PRDX6
Synonyms:1-Cys, AOP2, NSGPx, PRX, aiPLA2, p29, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Non-selenium glutathione peroxidase, Red blood cells page spot 12
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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