null

Human Peroxiredoxin 2 (PRDX2) ELISA Kit

SKU:
HUDL02392
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P32119
Sensitivity:
0.35ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
PRP,NKEFB,PRXII,TDPX1,TSA,Thioredoxin-Dependent Peroxide Reductase 1,Thiol-Specific Antioxidant 1,Natural Killer-Enhancing Factor B,Thioredoxin Peroxidase 1,Torin
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Peroxiredoxin 2 (PRDX2) ELISA Kit

The Human Peroxiredoxin-2 (PRDX2) ELISA Kit is specifically designed for the precise quantification of PRDX2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.PRDX2 is a key antioxidant enzyme that plays a critical role in protecting cells from oxidative stress and maintaining redox homeostasis. Dysregulation of PRDX2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for understanding disease pathology and potential therapeutic interventions.

With its reliable performance and ease of use, the Human PRDX2 ELISA Kit is an indispensable tool for researchers studying oxidative stress-related diseases and investigating the potential therapeutic strategies targeting PRDX2.

Product Name:Human Peroxiredoxin 2 (PRDX2) ELISA Kit
Product Code:HUDL02392
Size:96 Assays
Target:PRDX2
Synonyms:PRP, NKEFB, PRXII, TDPX1, TSA, Thioredoxin-Dependent Peroxide Reductase 1, Thiol-Specific Antioxidant 1, Natural Killer-Enhancing Factor B, Thioredoxin Peroxidase 1, Torin
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products