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Human Permeability Glycoprotein (Pgp) ELISA Kit

SKU:
HUDL02276
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
A6NDG6
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD243,ABCB1,ABC20,CLCS,GP170,MDR1,PGY1,P-Glycoprotein,Multidrug resistance protein 1,ATP-Binding Cassette,Sub-Family B(MDR/TAP),Member 1,Colchicin Sensitivity
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Permeability Glycoprotein (Pgp) ELISA Kit

The Human Permeability Glycoprotein (PGP) ELISA Kit is a reliable tool for detecting levels of PGP in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.PGP is a key protein involved in drug transportation and plays a vital role in drug resistance mechanisms in various diseases such as cancer and infectious diseases.

By measuring PGP levels, researchers can better understand drug permeability and develop strategies to overcome drug resistance in clinical settings.Overall, the Human Permeability Glycoprotein (PGP) ELISA Kit is an essential tool for studying drug transportation mechanisms and developing targeted therapies for drug-resistant diseases.

Product Name:Human Permeability Glycoprotein (Pgp) ELISA Kit
Product Code:HUDL02276
Size:96 Assays
Target:Pgp
Synonyms:CD243, ABCB1, ABC20, CLCS, GP170, MDR1, PGY1, P-Glycoprotein, Multidrug resistance protein 1, ATP-Binding Cassette,Sub-Family B(MDR/TAP),Member 1, Colchicin Sensitivity
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)85-10092
EDTA plasma (n=5)85-10293
Heparin plasma (n=5)87-9993
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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