Human pERK (Phospho Extracellular Signal Regulated Kinase) ELISA Kit
The Human PERK (Phospho-Extracellular Signal-Regulated Kinase) ELISA Kit is specifically designed for the accurate detection of phosphorylated extracellular signal-regulated kinase levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.PERK is a key protein involved in cellular signaling pathways, particularly in response to stress and the unfolded protein response.
Dysregulation of PERK activity has been linked to various diseases such as cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.With the Human PERK ELISA Kit, researchers can confidently measure phosphorylated extracellular signal-regulated kinase levels in human samples, advancing their understanding of cellular signaling pathways and potentially identifying new therapeutic targets.
Product Name:
Human pERK (Phospho Extracellular Signal Regulated Kinase) ELISA Kit
SKU:
HUES02690
Target:
Human pERK (Phospho Extracellular Signal Regulated Kinase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human pERK1/2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human pERK1/2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human pERK1/2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human pERK1/2. You can calculate the concentration of Human pERK1/2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
85-98
87-100
Average (%)
102
91
92
1:4
Range (%)
86-99
85-97
87-99
Average (%)
93
90
92
1:8
Range (%)
87-98
82-98
88-101
Average (%)
93
89
95
1:16
Range (%)
87-104
86-98
84-97
Average (%)
95
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.43
252.28
963.35
97.71
256.64
874.21
Standard deviation
5.1
14.56
36.13
6.08
10.6
43.36
C V (%)
5.46
5.77
3.75
6.22
4.13
4.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human pERK1/2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human pERK1/2 in samples. No significant cross-reactivity or interference between Human pERK1/2 and analogues was observed.
