The Human PER1 (Period Circadian Protein 1) ELISA Kit is designed to accurately measure PER1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and dependable results, making it a valuable tool for various research applications. PER1 is a key protein involved in the regulation of circadian rhythms, playing a crucial role in the synchronization of biological processes with the day-night cycle. Dysregulation of PER1 has been linked to various health conditions, including sleep disorders, mood disorders, and metabolic diseases.
Therefore, this ELISA kit provides researchers with a reliable method to study PER1 levels and potentially uncover new insights into these conditions. Whether you are investigating the impact of circadian rhythms on health, studying sleep disorders, or exploring potential therapeutic targets for metabolic diseases, the Human PER1 ELISA Kit from AssayGenie offers the precision and accuracy you need for your research endeavors.
Product Name:
Human Period Circadian Protein 1 (PER1) ELISA Kit
Product Code:
HUDL02260
Size:
96 Assays
Target:
PER1
Synonyms:
PER, RIGUI, hPER, Circadian clock protein PERIOD 1, Circadian pacemaker protein Rigui
Detection Method:
Sandwich
Range:
0.312-20ng/mL.
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
