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Human Perforin ELISpot Kit (HUDC0159)

SKU:
HUDC0159
Product Type:
ELISpot
Reactivity:
Human
€355

Description

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Human Perforin ELISpot Kit

Assay Genie ELISpot is a highly specific immunoassay for the analysis of Perforin production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of Perforin producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted Perforin (qualitative analysis) and single cells that produce Perforin (quantitative analysis). Cell secreted Perforin is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured Perforin is revealed by tracer antibodies and appropriate conjugates.

Human Perforin ELISpot Kit

The Human Perforin ELISPOT Kit is a powerful tool for analyzing the levels of perforin in human immune responses. This kit is specially designed to provide accurate and sensitive detection of perforin in peripheral blood mononuclear cells (PBMCs) or isolated immune cells. With its high sensitivity and specificity, researchers can rely on this kit to deliver consistent and trustworthy results.Perforin is a key protein involved in the immune response, primarily known for its role in cytotoxic T cell and natural killer cell-mediated killing of infected or abnormal cells.

By measuring perforin levels, researchers can gain insights into immune function and monitor immune responses in various diseases, including cancer, viral infections, and autoimmune disorders.Overall, the Human Perforin ELISPOT Kit is an essential tool for studying immune responses and evaluating immune function in various research settings. Its precision and reliability make it an invaluable asset for researchers looking to advance our understanding of immune-mediated diseases and develop potential therapeutic interventions.