null

Human Peptidylprolyl Isomerase C (PPIC) ELISA Kit

SKU:
HUDL02376
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P45877
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PPI-C,CYPC,Cyclophilin C,Peptidyl Prolyl Cis/Trans Isomerase C,Rotamase C
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Peptidylprolyl Isomerase C (PPIC) ELISA Kit

The Human Peptidylprolyl Isomerase C (PPIC) ELISA Kit is a reliable and accurate tool for detecting levels of PPIC in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures dependable and reproducible results, making it suitable for a variety of research applications.Peptidylprolyl Isomerase C (PPIC) is an important enzyme involved in protein folding and plays a role in various cellular processes. Dysregulation of PPIC has been associated with diseases such as cancer, autoimmune disorders, and neurodegenerative conditions.

Therefore, studying PPIC levels can provide valuable insights into these diseases and aid in the development of potential therapeutic interventions.Overall, the Human PPIC ELISA Kit offers researchers a powerful tool for investigating the role of PPIC in disease pathogenesis and identifying potential biomarkers for diagnostic and prognostic purposes.

Product Name:Human Peptidylprolyl Isomerase C (PPIC) ELISA Kit
Product Code:HUDL02376
Size:96 Assays
Target:PPIC
Synonyms:PPI-C, CYPC, Cyclophilin C, Peptidyl Prolyl Cis/Trans Isomerase C, Rotamase C
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products