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Human Peptidylglycine Alpha Amidating Monooxygenase (PAM) ELISA Kit

SKU:
HUDL02180
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19021
Sensitivity:
13.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PAL,PHM,Peptidylamidoglycolate lyase,Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase,Peptidylglycine Alpha-Hydroxylating Monooxygenase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Peptidylglycine Alpha Amidating Monooxygenase (PAM) ELISA Kit

The Human Peptidylglycine alpha-amidating monooxygenase (PAM) ELISA Kit is specifically designed for the accurate detection of PAM levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PAM is an important enzyme that plays a critical role in the amidation of bioactive peptides, contributing to various physiological processes such as hormone regulation and neurotransmitter synthesis.

Dysregulation of PAM activity has been implicated in conditions like hypertension, diabetes, and neurological disorders, making it a valuable biomarker for understanding these diseases and potentially developing targeted therapies.Overall, the Human PAM ELISA Kit provides researchers with a powerful tool for studying the role of PAM in health and disease, offering precise and accurate measurement of PAM levels in human samples.

Product Name:Human Peptidylglycine Alpha Amidating Monooxygenase (PAM) ELISA Kit
Product Code:HUDL02180
Size:96 Assays
Target:PAM
Synonyms:PAL, PHM, Peptidylamidoglycolate lyase, Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase, Peptidylglycine Alpha-Hydroxylating Monooxygenase
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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