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Human Peptidase D (PEPD) ELISA Kit

SKU:
HUDL02259
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P12955
Sensitivity:
0.63ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
PRD,Xaa-Pro Dipeptidase,Prolidase,Imidodipeptidase,Proline dipeptidase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Human Peptidase D (PEPD) ELISA Kit

The Human PEPD ELISA Kit is designed for the precise measurement of peptidase D (PEPD) levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PEPD is an essential enzyme involved in a wide range of biological processes, including peptide digestion and cellular homeostasis. Dysregulation of PEPD has been implicated in various diseases, highlighting its importance as a potential diagnostic and therapeutic target.

By utilizing the Human PEPD ELISA Kit, researchers can gain valuable insights into the role of PEPD in health and disease, paving the way for the development of innovative treatments and diagnostic tools. Trust in the reliability and performance of this kit to advance your research efforts in the field of peptidase D biology.

Product Name:Human Peptidase D (PEPD) ELISA Kit
Product Code:HUDL02259
Size:96 Assays
Target:PEPD
Synonyms:PRD, Xaa-Pro Dipeptidase, Prolidase, Imidodipeptidase, Proline dipeptidase
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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