Human PEDF (Pigment Epithelium Derived Factor ) CLIA Kit
The Human PEDF (Pigment Epithelium-Derived Factor) CLIA Kit is a highly sensitive and reliable assay designed for the accurate detection of PEDF levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it suitable for a variety of research applications.PEDF is a critical protein that regulates angiogenesis and acts as a neurotrophic factor, playing a key role in various physiological and pathological processes.
Dysregulation of PEDF has been implicated in conditions such as cancer, diabetic retinopathy, and neurodegenerative diseases, making it a valuable biomarker for studying these disorders and exploring potential therapeutic interventions.Overall, the Human PEDF CLIA Kit offers researchers a reliable tool to study the role of PEDF in various biological processes, making it an essential component of any laboratory studying angiogenesis and neurodegeneration.
Product Name:
Human PEDF (Pigment Epithelium Derived Factor ) CLIA Kit
SKU:
HUES01037
Target:
Human PEDF (Pigment Epithelium Derived Factor )
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PEDF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PEDF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PEDF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PEDF. You can calculate the concentration of Human PEDF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
84-99
98-110
Average (%)
99
90
104
1:4
Range (%)
103-117
86-98
94-109
Average (%)
110
91
101
1:8
Range (%)
88-101
99-112
97-114
Average (%)
94
106
104
1:16
Range (%)
87-98
92-104
93-105
Average (%)
93
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-117
107
EDTA plasma (n=5)
95-112
102
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.67
216.84
818.05
87.3
218.9
884.23
Standard deviation
7.86
20.79
59.15
8.21
16.07
96.29
C V (%)
8.22
9.59
7.23
9.4
7.34
10.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PEDF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PEDF in samples. No significant cross-reactivity or interference between Human PEDF and analogues was observed.
