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Human PDIA6 Recombinant Protein (RPPB2088)

SKU:
RPPB2088
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15084
Research Area:
Enzymes
€309
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Description

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Product Name:Human PDIA6 Recombinant Protein
Product Code:RPPB2088
Size:10µg
Species:Human
Target:PDIA6
Synonyms:Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, PDIA6, ERP5, P5, TXNDC7.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PDIA6 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 50mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKVGAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSSGKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLAAVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDL FSDNAPPPEL LEIINEDIAKRTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMKFALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL
Biological Activity:Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of INS in the presence of DTT.

PDIA6 belongs to the protein disulfide isomerase family (PDI). PDIA6 is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes which catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. PDIA6 functions as a chaperone that inhibits aggregation of misfolded proteins. PDIA6, also has a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.

PDIA6 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 442 amino acids (20-440 a.a.) and having a molecular mass of 48.5kDa. The PDIA6 is purified by proprietary chromatographic techniques.

UniProt Protein Function:PDIA6: May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. Belongs to the protein disulfide isomerase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 5.3.4.1; Isomerase

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; plasma membrane; ER-Golgi intermediate compartment

Molecular Function:protein binding; protein disulfide isomerase activity

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; protein folding; unfolded protein response; cell redox homeostasis; apoptotic cell clearance

NCBI Summary:Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM, Mar 2008]
UniProt Code:Q15084
NCBI GenInfo Identifier:2501205
NCBI Gene ID:10130
NCBI Accession:Q15084.1
UniProt Secondary Accession:Q15084,Q53RC7, Q6ZSH5, Q99778, B3KY95, B5MCQ5, B7Z254 B7Z4M8, F8WA83,
UniProt Related Accession:Q15084
Molecular Weight:440
NCBI Full Name:Protein disulfide-isomerase A6
NCBI Synonym Full Names:protein disulfide isomerase family A, member 6
NCBI Official Symbol:PDIA6��
NCBI Official Synonym Symbols:P5; ERP5; TXNDC7��
NCBI Protein Information:protein disulfide-isomerase A6; ER protein 5; protein disulfide isomerase P5; endoplasmic reticulum protein 5; thioredoxin domain-containing protein 7; protein disulfide isomerase-associated 6; protein disulfide isomerase-related protein; thioredoxin doma
UniProt Protein Name:Protein disulfide-isomerase A6
UniProt Synonym Protein Names:Endoplasmic reticulum protein 5; ER protein 5; ERp5; Protein disulfide isomerase P5; Thioredoxin domain-containing protein 7
Protein Family:Protein disulfide-isomerase
UniProt Gene Name:PDIA6��
UniProt Entry Name:PDIA6_HUMAN

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