Human PDGF-AA (Platelet Derived Growth Factor AA) ELISA Kit
The Assay Genie Human PDGF-AA ELISA Kit is meticulously designed for the sensitive and quantitative measurement of Platelet-Derived Growth Factor AA (PDGF-AA) levels in various biological samples. PDGF-AA, a crucial cell growth factor, plays a significant role in cell proliferation, wound healing, angiogenesis, and tissue repair processes. Accurate quantification of PDGF-AA is essential for understanding its role in various cellular functions and its implications in conditions related to growth regulation and tissue repair. Our PDGF-AA ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research endeavors.
Manufactured under stringent quality control standards, this kit guarantees robust performance and ease of use, making it an ideal choice for studies focusing on cell growth, angiogenesis, and related mechanisms. Trust Assay Genie's PDGF-AA ELISA Kit for precise and dependable quantification of PDGF-AA, empowering your scientific exploration and discovery in the field of growth factor biology.
Product Name:
Human PDGF-AA (Platelet Derived Growth Factor AA) ELISA Kit
SKU:
AEES00183
Target:
Human PDGF-AA (Platelet Derived Growth Factor AA)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-AA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-AA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-AA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PDGF-AA. You can calculate the concentration of Rat PDGF-AA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
90-102
83-95
Average (%)
102
97
90
1:4
Range (%)
97-113
89-102
85-100
Average (%)
103
96
92
1:8
Range (%)
93-103
87-101
86-101
Average (%)
98
93
93
1:16
Range (%)
96-111
87-100
80-94
Average (%)
103
93
86
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.64
7.17
22.41
2.77
6.96
23.0
Standard deviation
0.18
0.4
1.07
0.18
0.3
0.79
C V (%)
6.7
5.54
4.76
6.62
4.24
3.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PDGF-AA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGF-AA in samples. No significant cross-reactivity or interference between Rat PDGF-AA and analogues was observed.
