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Human PDE6D Recombinant Protein (RPPB2078)

SKU:
RPPB2078
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O43924
Research Area:
Enzymes
$371
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Description

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Product Name:Human PDE6D Recombinant Protein
Product Code:RPPB2078
Size:20µg
Species:Human
Target:PDE6D
Synonyms:Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, Phosphodiesterase 6D cGMP-Specific Rod Delta, GMP-PDE delta, Protein p17, PDE6D, PDED.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The PDE6D protein solution contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 1mM DTT and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH

Human PDE6D was initially identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. PDE6D is an Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain. PDE6D interacts with RPGR, ARL2 and ARL3. The catalytically active PDE6 is a heterodimer (?Beta) that is controlled by two inhibitory ? subunits. Since PDE6D does not change the catalytic properties of PDE?Beta, its function is still unanswered. PDE6D is expressed specifically in the retina.

PDE6D produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (1-150 a.a.) and having a molecular mass of 18.4kDa. PDE6D is fused to an 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 2q35-q36

Cellular Component: cytoplasmic vesicle; cytosol

Molecular Function:GTPase inhibitor activity; protein binding; Rab GTPase binding

Disease: Joubert Syndrome 22

NCBI Summary:This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]
UniProt Code:O43924
NCBI GenInfo Identifier:5921809
NCBI Gene ID:5147
NCBI Accession:O43924.1
UniProt Secondary Accession:O43924,O43250,
UniProt Related Accession:O43924
Molecular Weight:17,420 Da
NCBI Full Name:Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
NCBI Synonym Full Names:phosphodiesterase 6D
NCBI Official Symbol:PDE6D��
NCBI Official Synonym Symbols:PDED; JBTS22��
NCBI Protein Information:retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
UniProt Protein Name:Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
UniProt Synonym Protein Names:Protein p17
Protein Family:Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
UniProt Gene Name:PDE6D��
UniProt Entry Name:PDE6D_HUMAN

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