Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit
The Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit is specifically designed for the precise measurement of PDCD1LG1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research applications.PDCD1LG1 is a critical protein involved in immune responses, particularly in regulating T-cell activation and promoting T-cell apoptosis.
Dysregulation of PDCD1LG1 has been linked to autoimmune diseases, cancer, and other immune-related disorders, highlighting its importance as a potential biomarker for understanding and developing treatments for these conditions.This ELISA kit provides researchers with a reliable and efficient method for studying the role of PDCD1LG1 in various physiological and pathological processes, ultimately advancing our understanding of immune regulation and disease progression.
Product Name:
Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit
SKU:
HUES02561
Target:
Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PD-L1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PD-L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PD-L1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PD-L1. You can calculate the concentration of Human PD-L1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
90-100
85-98
Average (%)
104
95
90
1:4
Range (%)
88-99
84-95
86-101
Average (%)
93
90
93
1:8
Range (%)
87-99
83-94
87-101
Average (%)
94
89
94
1:16
Range (%)
90-102
79-92
85-100
Average (%)
96
85
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
102
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.35
4.76
0.49
1.29
5.23
Standard deviation
0.03
0.08
0.17
0.03
0.05
0.18
C V (%)
5.56
5.93
3.57
6.12
3.88
3.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PD-L1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PD-L1 in samples. No significant cross-reactivity or interference between Human PD-L1 and analogues was observed.
