Human PDCD1 (Programmed Cell Death Protein 1) CLIA Kit
The Human PDCD1 (Programmed Cell Death Protein 1) CLIA Kit is a cutting-edge diagnostic tool designed for the quantitative measurement of PDCD1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. PDCD1, also known as PD-1, is a key immune checkpoint protein that plays a critical role in regulating immune responses and maintaining immune tolerance. Dysregulation of PDCD1 expression has been implicated in various diseases, including cancer, autoimmune disorders, and infectious diseases.
The Human PDCD1 CLIA Kit provides researchers with valuable insights into the role of PDCD1 in disease pathogenesis and potential therapeutic interventions. Incorporating the latest technological advancements, the Human PDCD1 CLIA Kit from Assaygenie is an essential tool for researchers studying the immune system, cancer biology, and immunotherapy. Trust Assaygenie for high-quality and reliable research tools to advance your scientific discoveries.
Product Name:
Human PDCD1 CLIA Kit
SKU:
HUES00868
Target:
Human PD-1 (Programmed Cell Death Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PD-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PD-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PD-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PD-1. You can calculate the concentration of Human PD-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
87-97
91-107
Average (%)
108
92
98
1:4
Range (%)
87-100
91-106
86-100
Average (%)
93
97
93
1:8
Range (%)
93-106
94-105
84-97
Average (%)
100
99
91
1:16
Range (%)
103-116
89-102
100-115
Average (%)
110
96
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-117
108
EDTA plasma (n=5)
88-104
96
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.72
295.83
757.51
101.48
317.41
795.66
Standard deviation
8.91
25.26
57.87
8.92
38.06
62.38
C V (%)
9.41
8.54
7.64
8.79
11.99
7.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PD-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PD-1 in samples. No significant cross-reactivity or interference between Human PD-1 and analogues was observed.
