Human PCNA Associated Factor 15kDa (PAF) ELISA Kit
The Human PCNA-Associated Factor 15kDa (PAF) ELISA Kit is specifically designed for the precise and reliable detection of PAF levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.PAF is a critical protein that interacts with PCNA (proliferating cell nuclear antigen) and plays a crucial role in DNA replication and repair processes.
Dysregulation of PAF expression has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its significance as a potential biomarker for disease progression and therapeutic development.This ELISA kit offers researchers a valuable tool for studying the role of PAF in cellular processes and disease pathogenesis, ultimately advancing our understanding of key biological mechanisms and paving the way for novel therapeutic interventions.
Product Name:
Human PCNA Associated Factor 15kDa (PAF) ELISA Kit
Product Code:
HUDL02132
Size:
96 Assays
Target:
PAF OEATC1
Synonyms:
p15(PAF), L5, OEATC1, NS5ATP9, Overexpressed In Anaplastic Thyroid Carcinoma 1, Hepatitis C virus NS5A-transactivated protein 9
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
