The Human PCIII (Procollagen III) CLIA Kit is a highly reliable and accurate assay designed for the detection of Procollagen III levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Procollagen III is a key protein involved in collagen synthesis and tissue repair processes. Elevated levels of Procollagen III have been associated with various conditions such as liver fibrosis, chronic kidney disease, and cardiovascular disorders.
As such, this kit serves as a valuable tool for studying these conditions and potential therapeutic interventions.Overall, the Human PCIII (Procollagen III) CLIA Kit provides researchers with a reliable and efficient method for measuring Procollagen III levels, offering valuable insights into its role in disease pathogenesis and progression.
Product Name:
Human PCIII (Procollagen III) CLIA Kit
SKU:
HUES00156
Target:
Human PCIII (Procollagen III)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCâ…¢. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCâ…¢ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCâ…¢, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCâ…¢. You can calculate the concentration of Human PCâ…¢ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
102-115
104-118
Average (%)
92
108
110
1:4
Range (%)
93-105
96-109
87-99
Average (%)
100
103
93
1:8
Range (%)
100-114
103-117
94-106
Average (%)
108
109
100
1:16
Range (%)
89-104
88-105
92-105
Average (%)
96
96
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
98-114
106
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.22
278.42
968.85
97.88
272.76
949.65
Standard deviation
8.21
29.37
90.78
10.6
31.53
95.72
C V (%)
8.19
10.55
9.37
10.83
11.56
10.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCâ…¢ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCâ…¢ in samples. No significant cross-reactivity or interference between Human PCâ…¢ and analogues was observed.
