The Human PCI (Protein C Inhibitor) CLIA Kit is a cutting-edge assay designed for the precise measurement of protein C inhibitor levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees dependable and consistent results, making it an indispensable tool for various research purposes.Protein C inhibitor is a key player in the regulation of blood coagulation and inflammation, exerting its effects by inhibiting the proteolytic activity of activated protein C. Dysregulation of protein C inhibitor has been implicated in various pathological conditions, including thrombosis, sepsis, and inflammatory diseases.
Therefore, monitoring protein C inhibitor levels is crucial for gaining insights into these diseases and exploring potential therapeutic interventions.Don't miss out on the opportunity to explore the intricate world of protein C inhibitor with our state-of-the-art Human PCI CLIA Kit. Trust in its accuracy and reliability to propel your research forward and unlock new discoveries in the field of coagulation and inflammation.
Product Name:
Human PCI (Protein C Inhibitor ) CLIA Kit
SKU:
HUES00686
Target:
Human PCI (Protein C Inhibitor )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCI. You can calculate the concentration of Human PCI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
97-113
89-103
Average (%)
108
104
95
1:4
Range (%)
89-100
99-111
85-98
Average (%)
95
104
92
1:8
Range (%)
89-102
98-111
89-102
Average (%)
96
105
94
1:16
Range (%)
87-98
99-113
93-108
Average (%)
92
106
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-118
108
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.08
3.7
16.05
2.1
3.38
15.13
Standard deviation
0.17
0.36
1.2
0.26
0.33
1.29
C V (%)
8.17
9.73
7.48
12.38
9.76
8.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCI in samples. No significant cross-reactivity or interference between Human PCI and analogues was observed.
