The Human PCI (Procollagen I) ELISA Kit is specifically designed for the precise measurement of procollagen I levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Procollagen I is a key protein involved in the synthesis and formation of collagen, the main structural protein in the human body. As collagen is essential for maintaining the structural integrity of tissues and organs, abnormalities in its production can lead to various health conditions, such as bone disorders, skin aging, and connective tissue diseases.
Monitoring procollagen I levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With the Human PCI ELISA Kit, researchers can gain a deeper understanding of collagen synthesis and its impact on health and disease. This kit is a valuable tool for studying collagen-related disorders and exploring new treatment strategies.
Product Name:
Human PCI (Procollagen I) ELISA Kit
SKU:
HUES01448
Target:
Human PCI (Procollagen I)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCâ… . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCâ… and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCâ… , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PCâ… . You can calculate the concentration of Human PCâ… in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
85-98
88-101
Average (%)
97
91
95
1:4
Range (%)
89-105
85-97
86-100
Average (%)
96
91
92
1:8
Range (%)
90-104
79-92
86-98
Average (%)
95
85
92
1:16
Range (%)
89-101
83-93
89-101
Average (%)
95
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
212.5
533.08
1830.86
193.0
518.74
1967.5
Standard deviation
10.88
23.62
98.13
10.56
22.1
60.4
C V (%)
5.12
4.43
5.36
5.47
4.26
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PCâ… concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCâ… in samples. No significant cross-reactivity or interference between Human PCâ… and analogues was observed.
