The Human PCI Procollagen I CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of Procollagen I levels in human serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it ideal for a variety of research applications.Procollagen I is a key protein involved in the synthesis of collagen, which is crucial for maintaining the structure and strength of connective tissues such as skin, bone, and blood vessels. Abnormal levels of Procollagen I have been linked to conditions such as fibrosis, arthritis, and skin disorders, making it an important biomarker for studying these diseases and developing potential therapeutic interventions.
With the Human PCI Procollagen I CLIA Kit, researchers can accurately measure Procollagen I levels in biological samples, allowing for a better understanding of its role in disease pathology and the development of targeted treatments. This kit provides a reliable and efficient tool for advancing research in the field of collagen biology and related diseases.
Product Name:
Human PCI (Procollagen I) CLIA Kit
SKU:
HUES00155
Target:
Human PCI (Procollagen I)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCâ… . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCâ… and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCâ… , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCâ… . You can calculate the concentration of Human PCâ… in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
92-105
101-117
Average (%)
93
99
108
1:4
Range (%)
100-115
93-108
85-99
Average (%)
105
98
91
1:8
Range (%)
92-107
91-104
102-114
Average (%)
98
97
108
1:16
Range (%)
97-110
86-99
94-110
Average (%)
103
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.19
99.29
355.62
58.01
105.01
352.45
Standard deviation
5.91
8.77
22.83
5.86
7.77
24.95
C V (%)
11.11
8.83
6.42
10.1
7.4
7.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCâ… concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCâ… in samples. No significant cross-reactivity or interference between Human PCâ… and analogues was observed.
