Human PCBP1 Recombinant Protein (RPPB4193)
- SKU:
- RPPB4193
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q15365
Description
| Product Name: | Human PCBP1 Recombinant Protein |
| Product Code: | RPPB4193 |
| Size: | 20µg |
| Species: | Human |
| Target: | PCBP1 |
| Synonyms: | Poly(rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, PCBP1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PCBP1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC |
PCBP1 is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC. PCBP1 plays a role in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. PCBP1 along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. PCBP1 and PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.
PCBP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-163) and having a molecular mass of 19.6 kDa.PCBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. |
| UniProt Protein Details: | Protein type:Translation; RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 2p13-p12 Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane; nucleoplasm Molecular Function:mRNA binding; protein binding; RNA binding; single-stranded DNA binding Biological Process: gene expression; nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15365 |
| NCBI GenInfo Identifier: | 42560548 |
| NCBI Gene ID: | 5093 |
| NCBI Accession: | Q15365.2 |
| UniProt Secondary Accession: | Q15365,Q13157, Q14975, |
| UniProt Related Accession: | Q15365 |
| Molecular Weight: | 37,498 Da |
| NCBI Full Name: | Poly(rC)-binding protein 1 |
| NCBI Synonym Full Names: | poly(rC) binding protein 1 |
| NCBI Official Symbol: | PCBP1�� |
| NCBI Official Synonym Symbols: | HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1�� |
| NCBI Protein Information: | poly(rC)-binding protein 1 |
| UniProt Protein Name: | Poly(rC)-binding protein 1 |
| UniProt Synonym Protein Names: | Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; Nucleic acid-binding protein SUB2.3 |
| Protein Family: | Poly(rC)-binding protein |
| UniProt Gene Name: | PCBP1�� |
| UniProt Entry Name: | PCBP1_HUMAN |
