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Human PBK Recombinant Protein (RPPB2596)

SKU:
RPPB2596
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96KB5
Research Area:
Enzymes
$371
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Description

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Product Name:Human PBK Recombinant Protein
Product Code:RPPB2596
Size:20µg
Species:Human
Target:PBK
Synonyms:Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK.
Source:Escherichia Coli
Physical Appearance:DCK is supplied as a sterile filtered clear solution.
Formulation:PBK protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNV YLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPN IVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHG DIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLT LWEMMTLSIP HINLSNDDDD EDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV

Lymphokine-activated killer T-cell-originated protein kinase (PBK) is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase (MAPKK) family. PBK is abundant in placenta (is also found in the testis� outer cell layer of seminiferous tubules) and absent from adult brain tissue. PBK is involved in the activation of lymphoid cells and supports testicular functions, with a possible role in the process of spermatogenesis. Mitotic phosphorylation is required for PBK�s catalytic activity. Once phosphorylated,PBK forms a complex with TP53, leading to TP53 destabilization and weakening of G2/M checkpoint during�some kind of�DNA damage. PBK is active only during mitosis. In addition, a PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. PBK also phosphorylates MAP kinase p38, and may have a role in the activation of lymphoid cells.

PBK Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 346 amino acids (1-322 a.a) and having a molecular mass of 38.6kDa (Molecular weight on SDS-PAGE will appear higher).PBK is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
UniProt Protein Details:

Protein type:EC 2.7.12.2; Cancer Testis Antigen (CTA); Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; TOPK family

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nucleus

Molecular Function:ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: mitosis; negative regulation of inflammatory response; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation

NCBI Summary:This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:Q96KB5
NCBI GenInfo Identifier:83305809
NCBI Gene ID:55872
NCBI Accession:Q96KB5.3
UniProt Secondary Accession:Q96KB5,Q9NPD9, Q9NYL7, Q9NZK6, B4DX68, D3DST2,
UniProt Related Accession:Q96KB5
Molecular Weight:37,211 Da
NCBI Full Name:Lymphokine-activated killer T-cell-originated protein kinase
NCBI Synonym Full Names:PDZ binding kinase
NCBI Official Symbol:PBK��
NCBI Official Synonym Symbols:SPK; CT84; TOPK; HEL164; Nori-3��
NCBI Protein Information:lymphokine-activated killer T-cell-originated protein kinase
UniProt Protein Name:Lymphokine-activated killer T-cell-originated protein kinase
UniProt Synonym Protein Names:Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase
Protein Family:Lymphokine-activated killer T-cell-originated protein kinase
UniProt Gene Name:PBK��
UniProt Entry Name:TOPK_HUMAN

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