The Human Paxillin CLIA Kit is specifically designed for the precise measurement of paxillin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it ideal for various research applications.Paxillin is a key protein that plays a critical role in cell adhesion, migration, and signaling pathways. Dysregulation of paxillin has been implicated in various diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions.
Therefore, the Human Paxillin CLIA Kit serves as a valuable tool for studying these diseases and potentially developing new therapeutic interventions.Overall, the Human Paxillin CLIA Kit offers researchers a reliable and efficient method for analyzing paxillin levels, furthering our understanding of its role in health and disease.
Product Name:
Human Pax (Paxillin) CLIA Kit
SKU:
HUES01216
Target:
Human Pax (Paxillin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Pax. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pax and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pax, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Pax. You can calculate the concentration of Human Pax in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
99-113
97-115
Average (%)
94
104
105
1:4
Range (%)
94-107
87-98
88-100
Average (%)
102
92
94
1:8
Range (%)
85-99
97-109
91-106
Average (%)
92
103
97
1:16
Range (%)
99-113
91-102
95-108
Average (%)
106
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-117
107
EDTA plasma (n=5)
96-108
101
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.32
260.72
890.91
90.52
252.49
902.72
Standard deviation
11.92
19.87
84.64
10.4
22.85
80.07
C V (%)
12.38
7.62
9.5
11.49
9.05
8.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Pax concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pax in samples. No significant cross-reactivity or interference between Human Pax and analogues was observed.
