Human PARC/CCL18 (Pulmonary Activation Regulated Chemokine) ELISA Kit
The Human PARC (CCL18) ELISA Kit is specially designed for the precise measurement of pulmonary activation-regulated chemokine (PARC) levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit delivers accurate and consistent results, making it well-suited for a variety of research applications.PARC, also known as CCL18, is a chemokine that plays a key role in regulating inflammation and immune responses in the lungs. Elevated levels of PARC have been associated with various respiratory diseases, including asthma, chronic obstructive pulmonary disease (COPD), and pulmonary fibrosis.
Studying PARC levels can provide valuable insights into the mechanisms underlying these conditions and aid in the development of targeted therapies.The Human PARC (CCL18) ELISA Kit from Assay Genie is a valuable tool for researchers seeking to investigate the role of PARC in pulmonary diseases and explore potential therapeutic interventions. With its exceptional sensitivity and accuracy, this kit offers reliable results that can advance our understanding of respiratory health and disease.
Product Name:
Human PARC/CCL18 (Pulmonary Activation Regulated Chemokine) ELISA Kit
SKU:
HUES02328
Target:
Human PARC/CCL18 (Pulmonary Activation Regulated Chemokine)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PARC/CCL18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PARC/CCL18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PARC/CCL18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PARC/CCL18. You can calculate the concentration of Human PARC/CCL18 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
91-103
85-99
Average (%)
98
97
92
1:4
Range (%)
92-105
81-92
83-93
Average (%)
98
87
88
1:8
Range (%)
90-104
85-99
87-100
Average (%)
98
90
93
1:16
Range (%)
86-102
84-95
85-100
Average (%)
93
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
88-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
250.69
450.43
2496.99
246.2
435.82
2552.92
Standard deviation
16.95
23.87
93.39
14.48
18.17
121.26
C V (%)
6.76
5.3
3.74
5.88
4.17
4.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PARC/CCL18 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PARC/CCL18 in samples. No significant cross-reactivity or interference between Human PARC/CCL18 and analogues was observed.
