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Human Paraoxonase 2 (PON2) ELISA Kit

SKU:
HUDL02365
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15165
Sensitivity:
0.34ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
Serum Paraoxonase/Arylesterase 2,Paraoxonase Nirs,Aromatic esterase 2,Serum aryldialkylphosphatase 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Paraoxonase 2 (PON2) ELISA Kit

The Human Paraoxonase 2 (PON2) ELISA Kit is a reliable tool for the precise measurement of PON2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PON2 is an important enzyme that plays a key role in antioxidant defense and lipid metabolism. It is implicated in various diseases, including cardiovascular diseases, diabetes, and neurodegenerative disorders.

The measurement of PON2 levels can provide valuable insights into these conditions and aid in the development of novel therapeutic strategies.With its high-performance capabilities, the Human PON2 ELISA Kit is an essential resource for researchers investigating the biological functions and clinical significance of PON2. Trust in this kit for robust and precise analysis of PON2 levels in human samples.

Product Name:Human Paraoxonase 2 (PON2) ELISA Kit
Product Code:HUDL02365
Size:96 Assays
Target:PON2
Synonyms:Serum Paraoxonase/Arylesterase 2, Paraoxonase Nirs, Aromatic esterase 2, Serum aryldialkylphosphatase 2
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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