The Human Paired box gene 6 (PAX6) ELISA Kit is specifically designed for the accurate detection of PAX6 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PAX6 is a key transcription factor that plays a critical role in the development of the eye and central nervous system. Mutations in the PAX6 gene have been associated with various eye disorders including aniridia and Peters anomaly. The detection and quantification of PAX6 levels can provide valuable insights into the pathogenesis of these disorders, as well as potential therapeutic targets.
This ELISA kit provides researchers with a valuable tool for studying the role of PAX6 in development and disease, offering a reliable and efficient method for measuring PAX6 levels in human samples. Whether investigating ocular disorders, neurodevelopmental diseases, or other conditions related to PAX6, this kit is an essential resource for advancing research in this important area of biology.
Product Name:
Human Paired Box Gene 6 (PAX6) ELISA Kit
Product Code:
HUDL02196
Size:
96 Assays
Target:
PAX6
Synonyms:
AN, AN2, MGDA, WAGR, Oculorhombin, Aniridia,Keratitis, Aniridia type II protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
