The PAF ELISA Kit is designed for the quantitative detection of Platelet Activating Factor (PAF) in various human biological samples. PAF is a crucial bioactive lipid that plays a significant role in numerous biological activities. It mediates platelet aggregation and degranulation, promotes inflammation, and participates in processes such as hemostasis, thrombosis, and allergic reactions.
Accurate measurement of PAF is critical for understanding its mechanisms in disease conditions and for the development of therapeutic strategies. Assay Genie's PAF ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's PAF ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human PAF (Platelet Activating Factor) ELISA Kit
SKU:
AEES00033
Target:
Human PAF (Platelet Activating Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAF. You can calculate the concentration of Human PAF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
95-108
95-108
Average (%)
104
103
103
1:4
Range (%)
98-112
80-91
89-102
Average (%)
105
86
97
1:8
Range (%)
98-116
85-96
96-109
Average (%)
106
90
102
1:16
Range (%)
98-114
84-95
90-107
Average (%)
106
90
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
275.3
584.5
2496.8
279.4
549.5
2511.1
Standard deviation
16.5
33.3
104.9
17.0
25.8
100.4
C V (%)
5.99
5.7
4.2
6.08
4.7
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PAF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAF in samples. No significant cross-reactivity or interference between Human PAF and analogues was observed.
