Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit
The Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit is specifically designed for the precise measurement of PADI4 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring trustworthy and consistent results, making it a valuable tool for various research studies.PADI4 is an important enzyme involved in the citrullination of proteins, playing a critical role in autoimmune diseases such as rheumatoid arthritis and multiple sclerosis.
By accurately measuring PADI4 levels, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the Human PADI4 ELISA Kit is an indispensable tool for researchers studying autoimmune diseases and exploring the role of PADI4 in various physiological processes.
Product Name:
Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit
SKU:
HUES02373
Target:
Human PADI4 (Peptidyl Arginine Deiminase Type IV)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.11 ng/mL
Detection range:
0.19-12 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PADI4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PADI4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PADI4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PADI4. You can calculate the concentration of Human PADI4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
86-97
98-111
Average (%)
96
91
105
1:4
Range (%)
91-104
82-93
86-100
Average (%)
96
89
92
1:8
Range (%)
91-102
79-91
83-94
Average (%)
97
85
89
1:16
Range (%)
92-107
81-94
83-95
Average (%)
99
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.58
1.76
5.7
0.56
1.81
6.08
Standard deviation
0.03
0.09
0.21
0.03
0.09
0.26
C V (%)
5.17
5.11
3.68
5.36
4.97
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PADI4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PADI4 in samples. No significant cross-reactivity or interference between Human PADI4 and analogues was observed.
